P-34: Effect of Glucose on Buffalo Epididymal Sperm Motion Characteristics, Viability and Cytoplsmic droplets During 24 Hours Incubation
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Abstract:
Background: The recovery of sperm from the cauda epididymis may be the last chance to conserve, genetic Materials of valuable farm animals. Sperm utilize glucose as source of energy through glycolysis and oxidative phosphorylation to provide motility and movement. While endogenous glucose of the culture media has little potential to preserve sperm motility and viability for long period of time and it needs to replace or supplemented with exogenous glucose. So the aim of this study was to investigate the effect of adding glucose in culture medium on quality of buffalo epididymal spermatozoa during 24 hours incubation. Materials and Methods: Thirty testes from 15 mature buffalo bulls were collected from Urmia slaughterhouse and transported to laboratory in cool condition. Spermatozoa were obtained from caudal epididymis and pooled together. The samples were diluted in tissue culture media (TCM) at concentration of 20×106 sperm/ml and divided to five groups containing glucose at concentration of 0, 2.5, 5, 7.5 and 10 mM. Sperm motion characteristics, viability and cytoplsmic droplet were evaluated by computer assisted sperm analyzer and one steps eosinnigrosin stain respectively at 1, 6, 12 and 24 hours of incubation at 37 °C. Results: Sperm motion characteristics, viability and cytoplsmic droplets reduced in time depend manner. There were no significant differences in motion characteristics until 6 hours incubation. While addition of 5 and 7.5 mM glucose in culture medium increased motility and velocity patterns at 12 and 24 hours incubation, compared to control group (p<0.05). Distal cytoplsmic droplets were decreased significantly with supplementation of 5 and 7.5 mM glucose at 6 hour of incubation (p<0.05). Addition of 5 mM glucose resulted highest sperm viability at 6, 12 and 24 hours. Conclusion: Addition of 5 mM glucose to culture medium led to release cytoplsmic droplets and improves motility and viability of buffalo epididymal spermatozoa.
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Journal title
volume 7 issue 3
pages 49- 49
publication date 2013-09-01
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